FSC, SSC, and surface antigen expression of BM-and PB-derived CD34⁺ cells. (A) FSC and SSC display with 5 populations gated for further study. (B) Expression of CD13 versus c-kit and CD34 in each gate.1-5 The population of cells in gate 1 is CD34⁺/c-kit⁺/CD13⁺; gate 2 is mixed CD34⁺/c-kit⁺/c-kit⁻/CD13⁺/CD13⁻; gate 3 is mixed CD34⁺/CD34⁻/c-kit⁻/CD13⁻; gate 4 is CD34⁻/c-kit⁺/CD13⁺; and gate 5 is CD34⁻/c-kit⁻/CD13⁻. Results shown are representative of 6 experiments separately performed, using either BM (n = 3) or PB (n = 3) cells, and in which the FSC by SSC patterns resemble one another.