Fig. 6.
![Fig. 6. PP1 dose-dependently inhibits alboaggregin-A–induced tyrosine phosphorylation of FcR γ-chain, Syk, and PLCγ2.Platelets were preincubated for 3 minutes at 37°C with 0.25% dimethyl sulfoxide (DMSO) or various concentrations of PP1 before stimulation with alboaggregin-A (3.5 μg/mL) for 1 minute. Platelet suspensions were then lysed and either Syk or PLCγ2 immunoprecipitated. Precipitated proteins were then separated by SDS-PAGE and immunoblotted with 4G10 [A(i) and B(i)] and subsequently with either anti-Syk [A(ii)] or anti-PLCγ2 [B(ii)]. Immunoblots shown are representative of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/5/10.1182_blood.v94.5.1648/5/blod41731006w.jpeg?Expires=1722761923&Signature=k0kgnDOXKey~cINgXhuUlVBuV5bw9uT0LEuLU6LXq1qtJ7FfeC-w4U0jNLyYj8wfngSl746tRGacyO7D~fg5Ml9-nBYvw1heEvZZrOhxH8MEUj02u-TEyVgOFpXHKcvT2VuplhJxNsAhPXy~6fX5ScMZyRFCQSoIkGbWvJF0ZuxPGcqhUrBx6IVworq-lOE6K5~O93iCtO9w7M7C0PnrT48ixiY23cRyeceSBOMTI0U2FPIwXtCLng6AKilJ5bbiU2VXlQxRQKMVMxdR5V7uVCV9uWZTcKKJrIfBL1LKWNcxnr-91fXToG1iHTkQAWx3aig~OeWAQAJ6hF3rohkprQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PP1 dose-dependently inhibits alboaggregin-A–induced tyrosine phosphorylation of FcR γ-chain, Syk, and PLCγ2.Platelets were preincubated for 3 minutes at 37°C with 0.25% dimethyl sulfoxide (DMSO) or various concentrations of PP1 before stimulation with alboaggregin-A (3.5 μg/mL) for 1 minute. Platelet suspensions were then lysed and either Syk or PLCγ2 immunoprecipitated. Precipitated proteins were then separated by SDS-PAGE and immunoblotted with 4G10 [A(i) and B(i)] and subsequently with either anti-Syk [A(ii)] or anti-PLCγ2 [B(ii)]. Immunoblots shown are representative of 3 separate experiments.
