Coprecipitation of FcR γ-chain, GPIb, Lyn, and Fyn with GST-Syk-SH2 from platelets stimulated with alboaggregin-A.Platelets were either unstimulated (lane 1) or were stimulated with alboaggregin-A (3.5 μg/mL) for 15 seconds (lane 2) or 60 seconds (lane 3), and proteins were precipitated with 10 μg of GST-Syk-SH2 per lane. Precipitated proteins were then separated by SDS-PAGE and immunoblotted with GPIb, Lyn, Fyn, or FcR γ-chain, as indicated. Results shown are representative of 3 separate experiments.