Fig. 2.
![Fig. 2. Alboaggregin-A induces tyrosine phosphorylation of Syk and PLCγ2 and activates Syk kinase. For (A), Syk and for (C), PLCγ2 were immunoprecipitated from lysates of basal platelets (lane 1) or platelets stimulated with either alboaggregin-A (3.5 μg/mL; lane 2) for 1 minute or collagen (100 μg/mL; lane 3) for 2 minutes and Western blotted with either 4G10 [A(i) and C(i)] and anti-Syk [A(ii)] or anti-PLCγ2 [C(ii)]. For (B), a kinase assay was performed in vitro on Syk immunoprecipitates from basal platelets (lane 1) or platelets stimulated with alboaggregin-A (3.5 μg/mL; lane 2) for 1 minute, and presented as an autoradiograph. The results are representative of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/5/10.1182_blood.v94.5.1648/5/blod41731002w.jpeg?Expires=1724126697&Signature=MGCv~mXcWeS1vTEGl5lMRRv1iJRYFvHGgJY3-7-JZPpNeSzK2k10DHk7THA4vmUfFFHdfefaKSb2JN5-hlsUBgTkc3s1jmI1UIvaPQ2ErT9q1YFREn3MJ1~kKM-xMNOchgV5vV4wSaGZuo7aeOtsDYgqx-~wWzMlap1K57SyPutji5x9muHCfYPJy9R6Xrf89izpGIJFJ89w2aSX1GzqiYDjWokguQFoi9o8bgIZ4zuwtyNqGLwn5Vh0lSDweJPMB8e5VG6Ep5pIGH5BnzH~UlLjMcHkX~y8UY4qLrNhNqBCwQXYmNCQN1EV6ZDQtviyPlnllBMjUZctrDe~VVkn7g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Alboaggregin-A induces tyrosine phosphorylation of Syk and PLCγ2 and activates Syk kinase. For (A), Syk and for (C), PLCγ2 were immunoprecipitated from lysates of basal platelets (lane 1) or platelets stimulated with either alboaggregin-A (3.5 μg/mL; lane 2) for 1 minute or collagen (100 μg/mL; lane 3) for 2 minutes and Western blotted with either 4G10 [A(i) and C(i)] and anti-Syk [A(ii)] or anti-PLCγ2 [C(ii)]. For (B), a kinase assay was performed in vitro on Syk immunoprecipitates from basal platelets (lane 1) or platelets stimulated with alboaggregin-A (3.5 μg/mL; lane 2) for 1 minute, and presented as an autoradiograph. The results are representative of 3 separate experiments.
