BSAP/Pax5A-expressing EML cells successfully acquire the myeloid lineage commitment marker F4/80. B220⁺/F4/80⁻ EML cells were placed in culture at 3 × 10⁵ cells/mL and then induced to differentiate into B220^hi/F4/80⁺ and B220^lo/F4/80⁺ cells with 10% WEHI-3B conditioned supernatant (contains murine IL-3) and 10 μmol/L ATRA. Flow cytometry was performed on days 0 and 3.