Activation of IKK and IKKβ in human monocytic THP-1 cells by LPS derived from different gram-negative bacteria. Lane 1, Unstimulated cells; Lane 2, LPS from E coli 0127:B8 extracted by Boivin method (DIFCO); Lane 3, LPS from E coli 0127:B8 prepared by phenol extraction and gel filtration chromatography (Sigma); Lane 4, LPS from P aeruginosa serotype 10. LPS fromS minnesota. Human monocytic THP-1 cells were stimulated with different preparations of LPS at 1 μg/mL for 30 minutes and the IKK kinase assay was done as specified in Materials and Methods.The kinase complex was immunoprecipitated with anti-IKKβ antibody.