Fig. 1.
Fig. 1. Detection of IKK and IKKβ in human monocytic THP-1 cells by immunoprecipitation and immunoblotting. Cytoplasmic lysates from unstimulated cells were immunoprecipitated with anti-IKK NH2-terminal peptide antibody (lane 1) and anti-IKKβ COOH-terminal peptide antibody (lane 2). Immunoprecipitated proteins were resolved by (A) SDS-PAGE and immunoblotted with anti-IKK NH2-terminal peptide antibody, with (B) anti-IKKβ COOH-terminal peptide antibody (NS represents nonspecific band).

Detection of IKK and IKKβ in human monocytic THP-1 cells by immunoprecipitation and immunoblotting. Cytoplasmic lysates from unstimulated cells were immunoprecipitated with anti-IKK NH2-terminal peptide antibody (lane 1) and anti-IKKβ COOH-terminal peptide antibody (lane 2). Immunoprecipitated proteins were resolved by (A) SDS-PAGE and immunoblotted with anti-IKK NH2-terminal peptide antibody, with (B) anti-IKKβ COOH-terminal peptide antibody (NS represents nonspecific band).

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