FANCC and full-length FAZF interact in vivo. 293 EBNA cells were transiently transfected with epitope-tagged expression vectors for HA-FANCC and/or Flag-FAZF. Lysates were electrophoresed directly as WCE or after immunoprecipitation with anti-Flag epitope tag. Proteins were detected by immunoblotting with anti-Flag or anti-FANCC. (A) Lane 1, FANCC WCE; lane 2, FANCC + FAZF WCE; lane 3, FANCC + FAZF IP; lane 4, FAZF IP; lane 5, FAZF WCE; lane 6, FANCC IP; lane 7, pCEP-Flag WCE. (B) The same loading order as in (A), probed with anti-Flag.