[³H]Eicosanoid profile of stimulated PMN from AIDS subjects before and during therapy with GM-CSF. Prelabeled cells were incubated with 1 μmol/L A23187 for 15 minutes. HPLC elution profiles are displayed for [³H]AA metabolites synthesized by A23187-stimulated PMN from a representative HIV-infected subject on day 1 (A) and on day 4 (B). Peaks were identified by coelution with authentic standards, and the products were expressed as a percentage of incorporated radioactivity. This HPLC profile is representative of 6 subjects whose cells were radiolabeled.