Fig. 5.
![Fig. 5. [3H]Eicosanoid profile of stimulated PBM from AIDS subjects before and during therapy with GM-CSF. Cells prelabeled overnight were incubated with 1 μmol/L A23187 for 30 minutes. HPLC elution profiles are displayed for [3H]AA metabolites synthesized by A23187-stimulated PBM from a representative HIV-infected subject on day 1 (A) and on day 4 (B). Peaks were identified by coelution with authentic standards, and the products were expressed as a percentage of incorporated radioactivity. This HPLC profile is representative of 6 subjects whose cells were radiolabeled.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/11/10.1182_blood.v94.11.3897/4/blod42312005x.jpeg?Expires=1722806162&Signature=39MV3Ftcpoww1FjmR~sJyjfn2opGZXubsvZdRMrt6Tvxa~q7NJUhU08-nW0Iv8SnUCUAc3tMVQUxZshg8FgaODu-~HkReovzo-ksF-Z8XjbZUiJVcuumC2PVw6tIC71Blj~1lfJiHl1hqX1ah74ceAi-Cx3Lxmdrz-nrsFhixPkOSg9lFjTfi6A4NVqQp2NwXBVwEU8jQBYPxn7QCuulXUW79QD3rzqSINEEdzD~VL~qjW0ikTkH7TuCVp0HfF~DRgfZqwjMoTk4DReaLmrgf9mOc8UoH6uxCt6ijtmIOTd2ZcH900fOSs5jJPzkYK1C5PUuCZf7jQa68qEWqFI2JA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
[3H]Eicosanoid profile of stimulated PBM from AIDS subjects before and during therapy with GM-CSF. Cells prelabeled overnight were incubated with 1 μmol/L A23187 for 30 minutes. HPLC elution profiles are displayed for [3H]AA metabolites synthesized by A23187-stimulated PBM from a representative HIV-infected subject on day 1 (A) and on day 4 (B). Peaks were identified by coelution with authentic standards, and the products were expressed as a percentage of incorporated radioactivity. This HPLC profile is representative of 6 subjects whose cells were radiolabeled.
