 and β Caspase-8 (FLICE/MACH) isoforms are expressed in both Fas-susceptible and -resistant cell lines. First-strand cDNA was prepared from total RNA isolated from the indicated cell lines. Amplification reactions were performed with the indicated sense primers complimentary to sequences shared by both  and β isoforms and isoform-specific antisense primers. Products were then separated on 1.2% TAE-ethidium bromide agarose gels and photographed.