Chemotactic activity of mutated Tat proteins. (A) Inhibition of AS283 lymphoma cell motility by mutated Tat-MBP proteins. KGE-MBP and LxI-MBP proteins are mutated, respectively, in the RGD and basic domain of Tat. (B) AS283 lymphoma cell motility induced by a Tat protein mutated in the N-terminal domain. Each protein was tested at the concentration of 20 ng/mL. MBP or GST alone were used at the equimolar concentration. Data are expressed as the number of migrated cells in 20 high-power fields. Columns, mean of three replicates and representative of 1 out of 3 experiments; bars, SD. *P < .05 compared with corresponding control.