Effect of Rho-kinase inhibition and chelation of cytosolic Ca2+ on MLC-phosphorylation induced by different agonists. (a through d) MLC-phosphorylation levels in percent of the maximal increase upon stimulation of untreated controls. The maximal increase of MLC-phosphorylation stimulated by the different agonists was similar. Shown are controls (•), samples pretreated with 20 μmol/L Y-27632 (□), or 20 μmol/L BAPTA-AM and 2 mmol/L EGTA (▵). Values are mean ± SD of 3 to 4 independent experiments. (e) Autoradiographs of phosphorylated MLC after stimulation with 0.05 μg/mL CRP in untreated control samples (top), samples pretreated with 30 μmol/L Y-27632 (middle), or 50 μmol/L BAPTA-AM and 2 mmol/L EGTA (bottom). The results shown are representative of 2 experiments.
