Expression of XIAP and cIAP2 in JPX-9 cells. JPX-9 cells were preincubated with or without CdCl₂ for 6 hours, washed with PBS, and further incubated with or without PDTC (50 μmol/L), NAC (50 mmol/L), or LLL-CHO (10 μmol/L) for 24 hours. After incubation, the expression of XIAP and cIAP2 was examined as described in the text. Note that XIAP expression in CdCl₂-untreated JPX-9 cells is almost suppressed by NF-κB inhibitors, however, its expression in CdCl₂-treated JPX-9 cells is maintained even in the presence of NF-κB inhibitors. In contrast, cIAP2 expression in JPX-9 cells was not changed in each culture condition. Results are representative examples of 4 similar experiments.