Western blot pattern for CHEB LC and its mutants. Western blot analysis of LCs produced in vitro by transfected Sp2/0 cells. (A) Nonreduced conditions. Lane 1, CHEB chain; lane 2,CHEBm30; lane 3, CHEBm94. (B) Reduced conditions. Lane 1, CHEB chain; lane 2, CHEBm30; lane 3,CHEBm94. Molecular weight markers are indicated in kilodaltons. The difference in migration of molecular weight markers between (A) and (B) reflects nonreducing versus reducing conditions.