Fig. 8.
Enrichment for myeloid and erythroid CFCs in the lacZhigh fraction of fetal livers fromSCLlacZ/w mice. (A) Representative FACS profiles of forward scatter plotted against β galactosidase activity (measured as fluorescence) for FACS-Gal–labeled E12.5 fetal liver cells fromSCLlacZ/w and SCLw/w embryos. Sort windows and the percentage of cells in each window are shown for lacZlow and lacZhigh fractions for each genotype. (B) Frequency of d2 CFU-E and d7 BFU-E and myeloid (GM) CFC in cultures of unsorted and sorted fetal liver fractions fromSCLlacZ/w mice cultured in Epo (for d2 CFU-E) or IL-3/Epo (for d7 BFU-E and GM-CFC). Values represent the mean ± SD from replicate cultures of 10 fetal livers from 3 litters of embryos. The percentage of fetal liver cells, which were sorted into each fraction, is indicated.

Enrichment for myeloid and erythroid CFCs in the lacZhigh fraction of fetal livers fromSCLlacZ/w mice. (A) Representative FACS profiles of forward scatter plotted against β galactosidase activity (measured as fluorescence) for FACS-Gal–labeled E12.5 fetal liver cells fromSCLlacZ/w and SCLw/w embryos. Sort windows and the percentage of cells in each window are shown for lacZlow and lacZhigh fractions for each genotype. (B) Frequency of d2 CFU-E and d7 BFU-E and myeloid (GM) CFC in cultures of unsorted and sorted fetal liver fractions fromSCLlacZ/w mice cultured in Epo (for d2 CFU-E) or IL-3/Epo (for d7 BFU-E and GM-CFC). Values represent the mean ± SD from replicate cultures of 10 fetal livers from 3 litters of embryos. The percentage of fetal liver cells, which were sorted into each fraction, is indicated.

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