Stimulation of murine bEnd.3 cells with OSM increases mRNA for both P- and E-selectin. (A) Confluent monolayers of bEnd.3 cells were incubated in the presence or absence of 25 ng/mL murine OSM. To ensure that the effects of OSM were not caused by contaminating LPS, some cells were treated with OSM that was boiled to inactive the cytokine, or with 10 ng/mL of exogenously added LPS. After the indicated time, total RNA was isolated and analyzed by Northern blotting. (B) and (C) The OSM-induced increase in P- and E-selectin mRNA in each lane was quantified by densitometric scanning. The level of P- and E-selectin mRNA in each lane was normalized according to the level of CHO-B mRNA, which was not affected by OSM. The data are representative of 4 independent experiments.