eGFP expression in lineages. eGFP expression is shown for B lymphocytes (A) and T lymphocytes (B) in bone marrow, spleen, blood, and thymus from recipients of MNDeGFPSN-transduced and LeGFPSN-transduced (data for negative control LN not shown) bone marrow. Animals were killed and analyzed 2 weeks, 8 to 12 weeks, 4 to 5 months, and 6 to 8 months after BMT. Values for the percentage of cells showing expression were calculated by dividing the number of expressing lin⁺ cells by the total number of lin⁺ cells and are given as the mean ± standard error of the mean. n is the number of animals analyzed for each time point and vector. * and ** mark differences in the percentage of cells showing expression between MNDeGFPSN and LeGFPSN that are statistically significant (*P < .05 and **P < .005).