FACS analysis of eGFP expression. FACS dot blots and histogram blots from a representative pair of mice are shown. (A and B) Cells from bone marrow, spleen, thymus, and blood from recipients of LeGFPSN-transduced, MNDeGFPSN-transduced, and LN-transduced (data not shown) marrow were harvested and analyzed by FACS for eGFP expression (x axis) in granulocytes (Gr-1), monocytes/macrophages (MAC-1), B cells (B220), T cells (CD4, CD8), and RBC (Ter-119). Lineage-specific staining is shown on the y-axis. Quadrant statistics give the percentage of gated events. (C) Distribution of fluorescence intensity is shown in histogram plots for LeGFPSN (dark line) and MNDeGFPSN (solid area).