Induction of EBV-specific CTLs with peptide-pulsed DCs. (A) Peptide (FLRGRAYGL)-pulsed DCs induce HLA class I-restricted lysis of peptide-pulsed B8⁺ LCLs after 7 days of coculture. The CTLs did not lyse unpulsed HLA-matched LCL or peptide-pulsed B8⁻ LCL. The effector:target ratio was 20:1. (B) When the induction of CTL was relatively low, the response could be enhanced through the addition of IL-2 (with 50 IU/mL) on day 3 of the 7-day culture. (C) QAKWRLQTL-pulsed DCs induce HLA class I-restricted lysis of peptide-pulsed B8⁺ LCLs after 14 days of coculture. The culture was restimulated with peptide pulsed DCs on day 7 and IL-2 was added on days 1 and 7. Two effector:target ratios are shown (10:1 and 30:1).