Fig. 2.
PCR amplification of FXI mRNA from platelets and megakaryocytic cell lines. PCR was performed as described in the Materials and Methods section. (A) RT RNA extracted from human platelets was used as template to amplify exons 3-6 (top), exons 10-14 (middle), and exons 3-15 (bottom) of the FXI message. Lanes 1, RNA from 293 cells transfected with the human wild-type FXI cDNA (positive control); 2, RNA extracted from fresh platelets; 3 and 4, RNA extracted from platelets obtained by platelet-pheresis; and 5, no template (negative control). (B) RT poly-A RNA from megakaryocytic cell lines was used as template to amplify exons 3-6 of the FXI message. Lanes 1, HEL 92.1.7 cells; 2, MEG-01 cells; 3, CHRF-288-11 cells; and 4, no template (negative control). The positions of molecular weight markers (MWM) in kilobases are shown at the left of the figure.

PCR amplification of FXI mRNA from platelets and megakaryocytic cell lines. PCR was performed as described in the Materials and Methods section. (A) RT RNA extracted from human platelets was used as template to amplify exons 3-6 (top), exons 10-14 (middle), and exons 3-15 (bottom) of the FXI message. Lanes 1, RNA from 293 cells transfected with the human wild-type FXI cDNA (positive control); 2, RNA extracted from fresh platelets; 3 and 4, RNA extracted from platelets obtained by platelet-pheresis; and 5, no template (negative control). (B) RT poly-A RNA from megakaryocytic cell lines was used as template to amplify exons 3-6 of the FXI message. Lanes 1, HEL 92.1.7 cells; 2, MEG-01 cells; 3, CHRF-288-11 cells; and 4, no template (negative control). The positions of molecular weight markers (MWM) in kilobases are shown at the left of the figure.

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