Structure of the mvWF promoter. (A) Restriction map of an 8-kb region of the mvWF gene is shown. Exons I and II are depicted as thick and thin solid boxes, respectively. Introns and upstream promoter sequence are represented by thin lines. Restriction enzymes areKpn I (K), Pst I (P), Nco I (N), EcoRI (R), Xba I (X), Sac I (S), and EcoRV (RV). The numbers shown are relative to the start site of transcription. (B) Restriction fragments of P1 DNA or DNA derived from the tail of E129 mice were resolved on 0.8% agarose gel, transferred to a nylon membrane, and then hybridized with a ³²P-labeled probe derived from sequences within the first exon.