Ca²⁺ fluxes induced by factor VIIa in monocyte-derived macrophages. (A) Cells equilibrated for 10 minutes after fluo-3 loading. (B) Cells equilibrated for 15 minutes after Fluo-3 loading. Solid circles and continuous lines represent the response to addition of factor VIIa (2.5 μg/mL) added at 2 seconds (arrowed). Open circles and dotted lines represent the response to native factor VII (2.5 μg/mL). Triangles and broken lines represent responses to factor VIIa (2.5 μg/mL) when the cells were coincubated with anti-TF for 24 hours before the assessment of Ca²⁺fluxes.