Statistical evaluation of effect of Ca²⁺chelator dimethyl BAPTA, anti-GP Ib, or anti–GP IIb-IIIa on [Ca²⁺]i changes in the platelet adhesive and cohesive process on a vWF-coated surface under a shear rate of 1,500 s⁻¹. Experimental conditions were as described in the Fig3 legend. To evaluate the state of platelet adhesion, the number of translocating platelets, defined as those moving along the surface in a greater distance than the corresponding platelet diameter in 1 second (see Materials and Methods), within a defined area, was determined, in addition to the G/R ratio and surface coverage. Data represent the mean and SD obtained by analysis of 5 areas (4,760 μm² each) randomly selected from images derived from 5 independent perfusions (8 minutes at 1,500 s⁻¹). One-way factorial ANOVA and Scheffe’s method were used for analysis of variance and comparison of each agent against control, respectively, with assist of Stat View computer software. Asterisks (*) denote statistically significant differences from a control (P < .01). These statistical analyses support the findings obtained by visual recognition in Fig 3; dimethyl BAPTA inhibited both [Ca²⁺]i elevation and surface coverage without affecting firm adhesion of platelets, and clear inhibition of all 3 parameters was observed in the presence of anti–GP IIb-IIIa antibody.