Dose-response analyses of immunophenotypic modulations of cultured CD34^pos bone marrow cells during CI A23187 treatment. Six-day cultured CD34^pos myeloid cells enriched from normal volunteer bone marrow were cultured in medium containing rhGM-CSF and rhTNF- with graded doses of CI A23187 (0 to 750 ng/mL). Cells were harvested and analyzed by flow cytometry with added propidium iodide (PI) as described in Fig 6 and Methods. Abscissa displays ng/mL of A23187 present during CI treatment. Two or three separate dose-response experiments were performed and plotted for each cell surface marker analyzed (B7.1, B7.2, CD83, ICAM-1, HLA-DR, CD1a, and CD14). Also portrayed is the viable cell recovery relative to control for three dose-response experiments (0 ng/mL group = 100%). Cell surface Ag expression data are portrayed as Specific Mean Fluorescence quotients (mean fluorescent intensity of cells stained with specific Ab/mean fluorescent intensity of cells stained with subclass-matched control Ab); a minimum of 5000 cells with background-level PI staining was assayed per determination.