Fig. 7.
Induction of FasL in PBMCs after EBV or PHA stimulation. PBMCs from healthy blood donors were incubated at 1 × 106cells/200 μL for 2 hours on ice with 1/10 vol of mock virus, purified Akata virus, or a final culture dilution of 1:500 (vol/vol) PHA. Cells were washed 2 times with cold PBS and cultured for 24 hours at 5 × 105 cells/mL. Cells were incubated with PE-conjugated CD4, CD8, CD20, CD16, or CD14 and rabbit anti-FasL, followed by incubation with FITC-conjugated goat antirabbit IgG. Cells were analyzed by FACS analysis and the results are expressed as the mean percentage positive cells ± SEM for 5 separate blood donors.
