Binding of EBV to T and B cells (A and B, respectively). Affinity-purified T or B cells derived from a healthy donor’s peripheral blood (shown in upper right inserts) were incubated at 1 × 106 cells/200 μL on ice for 2 hours with 1/10 vol of concentrated B95-8 virus or mock virus stock preparations. Cells were washed with cold PBS and stained for bound gp350 using anti-gp350 MoAb, 2L10. Samples were developed with FITC-conjugated goat antimouse IgG. Results were plotted on a logarithmic scale. Virus- and mock-infected cells are indicated by solid or open histograms, respectively. The percentage of fluorescent cells greater than that of the mock-infected cells is indicated above the bar. T and B cells were 87% and 92% viable, respectively, after affinity purification, as measured by trypan blue exclusion.
