Levels of CD95 expression on PBMCs and CD4⁺and CD8⁺ peripheral blood T cells from AIM patients. PBMCs from a representative healthy blood donor (A through C) or from an AIM patient (D through F) were stained with FITC- and PE-conjugated control IgG (A and D), FITC-conjugated anti-CD95 and PE-conjugated anti-CD4 (B and E), or FITC-conjugated anti-CD95 and PE-conjugated anti-CD8 (C and F). Results from gated cells (upper right inserts in [A] and [D]) were plotted on a logarithmic scale. CD95 expression in gated peripheral blood lymphocytes from 25 healthy blood donors (□) or 7 AIM patients (▪) (G). Cells were analyzed by 2-color immunofluorescence after staining with FITC-conjugated CD95 (x-axis) and PE-conjugated anti-CD4 or anti-CD8 MoAbs (y-axis). Results are expressed as the mean percentage positive ± SD. Statistically significant values analyzed by the Mann-Whitney U test are designated by an asterisk.