Fig. 6.
![Fig. 6. MEK inhibition has no obvious effect on murine megakaryocyte morphology and acetylcholinesterase activity in whole marrow cultures. Murine bone marrow cells were harvested and split into 2 flasks. Both were grown in serum-free media with 35 ng/mL murine TPO for 3 days. One culture contained 50 μmol/L PD 98059 and the other contained an equal amount of DMSO. Cytospin preparations were then performed and stained with Giemsa ([A] DMSO; [B] PD 98059) or acetylcholinesterase activity (brown staining; [C] DMSO; [D] PD 98059).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/4/10.1182_blood.v94.4.1273/4/blod41604006ay.jpeg?Expires=1724239591&Signature=PQ8MdbEPPlMk1RLdMMubkifcU4f1BXbn3WuL6f6zbS5ILvLxFjqHhS9YeOiAxvnd3H2lFJGZAsMRrptkwsogOZ1PVE4eKCtrdIKp-okWRtmYQtklXWNg0QX34JCG~2a~6I59HEaro6dekNbG1jMlZDSuTBIHbDAi9PM6zeGz9uMRQzXjUN8n1SjxErBHDX46brn6uXq-EmrxVFjp7aUXyMyPhg6MbfbZRGvSmQ1LJCCAi~lTqGOTZW~HxW5zVhBSuCoZs-zvqt4UtfVtKGuowPf1JdSRxr8Ky5G1cf56BPD~D7y~0Rb3LZkltLbkbq0Um99LlXMTNqrcQNoGm4ZKcw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
MEK inhibition has no obvious effect on murine megakaryocyte morphology and acetylcholinesterase activity in whole marrow cultures. Murine bone marrow cells were harvested and split into 2 flasks. Both were grown in serum-free media with 35 ng/mL murine TPO for 3 days. One culture contained 50 μmol/L PD 98059 and the other contained an equal amount of DMSO. Cytospin preparations were then performed and stained with Giemsa ([A] DMSO; [B] PD 98059) or acetylcholinesterase activity (brown staining; [C] DMSO; [D] PD 98059).
