Fig. 1.
Fig. 1. In utero transplant of WT hematopoietic progenitors. E14.5 embryos (Ly 5.2) from PU.1+/− matings were injected with 105 WT AA4.1+ HPC (Ly 5.1). The resulting animals were then genotyped by Southern blot at 3 weeks of age. At 6 weeks of age, bone marrow underwent 2-color flow cytometric analysis for B-cell (B220+/Ly 5.1+) and monocyte (CD11b+/Ly 5.1+) origin, and the thymus was analyzed for T-cell (CD4+/Ly 5.1+) origin.

In utero transplant of WT hematopoietic progenitors. E14.5 embryos (Ly 5.2) from PU.1+/− matings were injected with 105 WT AA4.1+ HPC (Ly 5.1). The resulting animals were then genotyped by Southern blot at 3 weeks of age. At 6 weeks of age, bone marrow underwent 2-color flow cytometric analysis for B-cell (B220+/Ly 5.1+) and monocyte (CD11b+/Ly 5.1+) origin, and the thymus was analyzed for T-cell (CD4+/Ly 5.1+) origin.

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