Fig. 4.
![Fig. 4. Effect of MCP-1 on intracellular [Ca2+]i in differentiating monocytes. Cells (5 × 106/mL) were incubated with Indo-1 probe (2 μg/mL final) at 37°C for 30 minutes, washed, and exposed in cuvette (1 × 106/mL) to recombinant MCP-1 (10 ng/mL) or RANTES (10 ng/mL) or FMLP (1 × 10−7 mol/L) in the presence of 1 mmol/L extracellular Ca2+. One representative experiment of 3 is shown in (A). (B) Average values obtained in 3 independent experiments with monocytes from different donors.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/3/10.1182_blood.v94.3.875.415k28_875_883/5/blod41528004x.jpeg?Expires=1723321567&Signature=37sEVIQycNiNLnryJwh6QZPuNljLjbMvIRYVRJJavWFJIRFsojASh9cOzz3UaxdXb3xTr7vH2Di8moVjqTNFZrMyxmG9rhr0QGBEDIJ-cu~lPNbf1IJvppxcCsNeV2-V9SAc4P7m4qPj29Xl-zlGJB~z-~BKP5Tyum1ggMEx4PD5BIRl2QQkz6RoF2g2LvN6Hj4-1pZjKQRUb8~DuSEyUETM1pNLJPezOwde9PXZp85BX8fJjeFQLKVeZ7nXevWVyC40LyOMmxELGQDEdTV1yKxt~GRt-i9Co7J5HGih82c-xO3jwIuJUfyQeXgZBZyDaTU35CWH7JZfSLS2fMCQQA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of MCP-1 on intracellular [Ca2+]i in differentiating monocytes. Cells (5 × 106/mL) were incubated with Indo-1 probe (2 μg/mL final) at 37°C for 30 minutes, washed, and exposed in cuvette (1 × 106/mL) to recombinant MCP-1 (10 ng/mL) or RANTES (10 ng/mL) or FMLP (1 × 10−7 mol/L) in the presence of 1 mmol/L extracellular Ca2+. One representative experiment of 3 is shown in (A). (B) Average values obtained in 3 independent experiments with monocytes from different donors.
