Sensitization of H9 cells for cell death induced by Fas/CD95, CD3− crosslinking, or dexamethasone. (A) Activation-induced cell death on CD3 cross-linking by immobilized anti-CD3 monoclonal antibody (MoAb) (clone OKT3). Plates were coated with OKT3 (coating concentration 0.1 to 100 μg/mL) as described.14,17 (B) Induction of cell death by CD95/Fas triggering by (soluble) anti-CD95 MoAb (clone anti-APO-1 IgG3). (C) Cell death induction by dexamethasone, which was added to the cultures at concentrations from 10−8 mol/L to 10−5mol/L. Cell death was determined on the single-cell level by measuring the DNA content of individual nuclei by flow cytometry. Data represent the mean of triplicates ± SD. (A through C) H9 control cells (○), Bik/Nbk clone no. 2 (◍), Bik/Nbk clone no. 10 (▩), Bik/Nbk clone no. 16 (▴). Medium control in (A) or cultures incubated with FII23c isotype-matched control antibody (0.12 μg/mL19) in (B): H9 control cells (◊), Bik/Nbk clone no. 2 (✙), Bik/Nbk clone no. 10 (□), Bik/Nbk clone no. 16 (▵).
