Effect of PF-4–derived peptides on binding of¹²⁵I-FGF-2 to endothelial cells. LEII cells or ACE cells are incubated with 10 ng/mL ¹²⁵I-FGF-2 in the presence or absence of 2 μg/mL unlabeled ligand (nonspecific binding, NS), 10 μg/mL PF-4 or PF-4–derived peptides. Binding experiments are performed as indicated in Materials and Methods. Binding experiments on LEII cells with increasing concentrations (A) of peptides 47-70 (•) or 34-58 (▪) or fixed concentrations (B, 10 μmol/L) of the indicated peptides; binding experiments on ACE cells with fixed concentrations (C, 10 μmol/L) of indicated peptides. Results are expressed as the mean± SD of experiments done in duplicates.