Fig. 3.
Quantitative analysis of SRC after 10 to 14 days of ex vivo cultures. (A) CB CD34+ cells were transplanted into NOD/SCID mice (105 cells/mouse) from replicate wells. The cells in the wells were ex vivo-cultured for 6 or 11 days with SCF + FL + IL6RIL6 chimera. At each time point, the content of 1 well containing initial cells and their expanded progeny was transplanted into a mouse. DNA was extracted from the BM of transplanted mice 1 month after transplantation and hybridized with a human-specific probe. (B) A summary of the levels of human engraftment in mice transplanted with CD34+ cells that were ex vivo-cultured for 10 to 14 days before transplantation. Data shown are from 4 independent experiments.
