LRP and mannose receptor expression in livers from control and EE-treated mice. Membrane fragments were isolated from livers of mice treated for 4 days with vehicle (−) or EE. Solubilized membrane proteins were subjected to SDS-PAGE and transferred to a nitrocellulose membrane, as described in Materials and Methods. LRP (A) was visualized by incubating the blot with ¹²⁵I GST-RAP. MR (B) was incubated with an MoAb against the MR and visualized as described in Materials and Methods. Equal loading was controlled for by Ponceau coloring of the blots.