Complete amino acid sequence of trocarin. Strategy for determination of the sequence of light (A) and heavy chains (B). N-term.: Amino terminal sequence of intact chain. Peptides were derived from a digest of light or heavy chains by endoproteinase Lys C (Lys C), endoproteinase Glu C (Glu C), endoproteinase Asp N (Asp N), cyanogen bromide (CNBr), and BNPS-skatole (BNPS-sk). Solid lines represent regions whose sequences were determined by Edman degradation and/or confirmed by mass spectrometry. Broken lines represent regions of peptides that were not sequenced.