Northern blot analysis of gp91^phoxexpression in murine neutrophils and macrophages. Total cellular RNAs (5 μg per lane) were electrophoresed on a denaturing gel, transferred to a nylon membrane, and probed with a radiolabeled murine gp91^phox cDNA. The position of the 28S and 18S ribosomal RNAs are indicated. The arrows indicate the position of the unspliced and spliced LTR-driven transcripts. (A) RNA was extracted from BM neutrophils from wild-type mice (WT), X-CGD mice (CGD), and X-CGD mice 11 months (mice 26, 27) and 18 months (mice 11, 12) posttransplantation with MSCV-m91Neo–transduced BM. The lower panels show the ethidium bromide staining of the 28S and 18S ribosomal RNAs (rRNAs). (B) RNA was extracted from peritoneal exudate macrophages from wild-type mice, X-CGD mice, and X-CGD mice 8 months posttransplantation with MSCV-m91–transduced BM. The lower panel shows hybridization of the same blot reprobed with radiolabeled actin cDNA.