Antigen presentation by hFcγRIa-transfected IIA1.6 cells. (A) Cells were incubated with rabbit IgG-ovalbumin complexes (10 or 100 ng/mL) or OVA alone (100 ng/mL or 100 μg/mL) for 6 hours, washed, and incubated with ovalbumin-specific T cells for 16 hours at 37°C. IL-2 released by T cells was determined by CTLL proliferation assays. Data represent means of duplicate determinations in 1 representative experiment of 8. (B) Antigen presentation observed in 8 individual experiments was compared by calculating antigen presentation indices (API). API were defined by dividing cpm of incorporated3H-thymidine obtained in the presence of complexes by background cpm (obtained in the absence of complexes). Error bars represent standard errors of the mean.
