Expression of E-selectin in inflamed tissue. Immunohistochemical staining of serial sections at the junction between viable and necrotic tissue indicated by the dotted line. Sections were stained with antibodies against PECAM-1 (A), Mac-1 (B), and E-selectin (C). There is intense neovascularization at the interface of viable and necrotic tissue (A) and infiltration of Mac-1–positive leukocytes (neutrophils and monocytes) in the necrotic tissue (B). Despite this indication of active inflammation, no significant expression of E-selectin was detected on any of the vessels in this tissue (arrows).