Expression of endothelial cell adhesion molecules in inflamed tissues. Serial tissue sections from visually and histologically inflamed ulcerated tissue removed at the time of the patient’s below-the-knee amputation were stained immunohistochemically with antibodies against PECAM-1 (A), ICAM-1 (B), P-selectin (C), and E-selectin (D). Two vessels (long and short arrows) are identified by staining with antibody against PECAM-1. Although ICAM-1 expression (B, long arrow) and P-selectin expression (C, short arrow) were detected on the vasculature, no expression of E-selectin was detected on these or other vessels in the surrounding tissue. Staining with 3 anti–E-selectin antibodies gave similar results.