Chromosomal assignment of the human EPCR gene. A human monochromosomal somatic cell hybrid DNA panel and controls were amplified by PCR to determine the chromosomal location of the EPCR gene. The exon-specific primers EPCR-3 and EPCR-4 (Table 1) were used and the products were separated on a 1% agarose gel. The templates in lanes 1 to 24 were mouse/human or hamster/human somatic cell hybrids containing chromosomes 1 to 22, X and Y chromosomes, respectively. Lanes 25 and 28 were human genomic DNA samples; one was provided with the panel (lane 25) and one was our internal control (lane 28). Lanes 26 and 27 were mouse and hamster genomic DNA, respectively. The marker lane (M) is pHC624/Taq I-pMJ/Nci I (Advanced Biotechnologies, Leatherhead, UK). This contains fragments of 1444, 696, 475, 411, 358, 352, 212, and 96 bp (the 358- and 352-bp fragments comigrate).