Double immunolabelling and LSCM studies. A and B. Reactive Lymphoid Tissue. (A) Tonsil surface epithelium: cyclin D3 expression (red) in the lower rds and p27 expression (green) in the upper third. Cyclin D3 and p27 colocalization (yellow) is very unusual. (B) Tonsil reactive follicle showing cyclin D3 expression (red) mainly restricted to germinal center cells and p27 expression in mantle cells and very few germinal center cells (green). Cyclin D3 and p27 colocalization is very infrequent (yellow). C, D, and E. DLBCL with high (60%) cyclin D3 expression (red) (C) and moderate (20%) p27 expression (green) (D). Cyclin D3 and p27 colocalization (E) (yellow) is observed in isolated cells. F, G, and H. DLBCL with (F) high (79%) cyclin D3 expression and (G) p27 overexpression (85%). Cyclin D3 and p27 colocalization (H) occurs in almost every cell.
