Effect of serum and bFGF on nuclear run-on transcription of TFPI gene. Quiescent monolayers of PASMC were treated with control basal medium (Control), bFGF/heparin (10 ng/15 U/mL), or serum (10% vol/vol) for 8 hours. Three identical blots containing TFPI and other target DNAs were hybridized with equal amounts of labeled transcripts of nuclear RNA. DNAs used were as follows: -tubulin (-Tub), TFPI, urokinase-type plasminogen activator receptor (uPAR), urokinase-type plasminogen activator (uPA), tissue-type plasminogen activator (tPA), and TF.