CLSM of immunofluorescence staining of eosinophils. (A through C) Unstimulated eosinophils labeled with BODIPY FL indicating RANTES immunoreactivity (A), Texas Red corresponding to MBP (B), and combined images (C). (D through F) Higher magnification of eosinophil crystalloid granules showing matrix-associated doughnut-shaped RANTES immunoreactivity (D), surrounding red-labeled cores of MBP immunoreactivity (E), and combined images of the same structure (F). (G through L) Combined images of RANTES and MBP, depicting time course of IFN-γ (500 U/mL) stimulation, comparing (G) unstimulated cells with those stimulated for 5 minutes (H), 10 minutes (I), 30 minutes (J), 60 minutes (K), and 16 hours (L). (M) Lower magnification of combined images of eosinophils stimulated for 10 minutes with IFN-γ. (N) Single-labeled unstimulated eosinophil, compared with (O) cell after 10 minutes stimulation (500 U/mL IFN-γ). (P) Inhibitory effect of 10⁻⁶ mol/L genistein added for 10 minutes before IFN-γ stimulation. (Q) Cell incubated with 1,000 U/mL IFN- for 10 minutes. Original magnification × 100 for all images, except for (M), ×63.