Size distribution of thrombi formed on type I collagen fibrils at different wall shear rates. Washed blood cells were perfused after suspension in buffer only (Control) or with the addition of fibrinogen (Fg) or vWF, individually or concurrently as indicated. The concentration of fibrinogen was 2 mg/mL, and the concentration of vWF was 20 μg/ml (see Fig 3). At the indicated time points, a single image was obtained from the arithmetic sum of all those in a confocal series, and the area of thrombi thus projected on the surface was determined. Thrombus size was arbitrarily classified as small when the latter value was less than 300 μm2, and large when it was greater than 300 μm2. Height and shape were not considered in these definitions; thus, the classification is not directly related to volume. The results shown are the mean ± SEM of four to seven experiments performed for each condition tested.
