(A) Autoradiographic image of vWF subunit (225 kD) and fragments (189, 176, and 140 kD) detected with a pool of MoAbs directed against different epitopes of vWF. Lane 1, normal plasma pool; lane 2, fragmentation of the protein in a recurrent patient (R7) during the acute phase; lane 3, the same patient at remission; lane 4, increased fragmentation in a patient with the familial form (F64#5) collected during remission. (B) Autoradiographic image obtained using MoAb anti N terminus (that labels in normal plasma the 140-kD fragment, white arrow). Lane 1, normal plasma pool; lane 2, a recurrent patient (R29, acute phase) showing an abnormal band at the 176-kD position (black arrow); lane 3, a patient with the familial form at remission (F45#2) with a similar abnormal fragment. (C) Representative image obtained using MoAb anti-C terminus (that labels in normal plasma 189- and 176-kD fragments, white arrows). Lane 1, normal plasma pool; lane 2, a recurrent patient (R12) showing both normal fragments and an abnormal fragment as shown by the appearance of a band of 140 kD (black arrow, not detected in normal plasma with this MoAb); lane 3, a patient with the familial form (F108#24 at remission) showing an abnormal band at 150 kD (black arrow) in addition to normal fragments.