Fig. 1.
Localization of cells expressing c-fmsmRNA in the embryo. In all panels, c-fms mRNA was localized by whole mount in situ hybridization as described in Materials and Methods. The blue-purple reaction product indicates sites of expression of the c-fms mRNA. In panels F, G, and H, the stained embryos were embedded, sectioned, and counterstained with neutral red. Bar in panels A through C and E represents 250 μm; in panels D, F through H, 20 μm; heart (*), forelimb (fl), hindlimb (hl), neural tube (nt). (A) Embryo 10.5 dpc. Localization of c-fms mRNA shows a very extensive speckled pattern throughout the embryo. Each spot is a single-labeled cell. Labeled cells are particularly concentrated in the head, around the branchial arches, and along the dorsal midline, although this is not evident in a single focal plane. (B) Embryo 11 dpc. The head of the embryo was removed before staining. At a slightly more advanced stage than (A), the rapid appearance ofc-fms–labeled cells is evident in the liver (l). The fine speckled pattern of labeled cells is more prominent throughout the embryo than in (A), but is less readily demonstrated in a single focal plane. (C) Hindlimb of 12.5 dpc embryo showing accumulation ofc-fms–positive cells at the apical ectodermal ridge (aer) (arrowheads) and infiltration between the digits (arrows). (D) A section through the limb bud of 12.5 dpc embryo showing the positive cells leaving the marginal sinus (ms) and infiltrating the mesenchyme. (E) Whole mount of the head of a hemisected 12.5-dpc embryo. Numerousc-fms–positive cells are lining the ventricular surfaces of the brain, including telencephalon (tel), diencephalon (dic), midbrain (mb), and hindbrain (hb). (F) Section through the body wall of an 11.5-dpc embryo. Numerous c-fms–positive cells accumulate particularly along the dorsal midline (d). In this field, they can be seen to be closely associated with the vascular spaces adjacent to the neural tube, but are also infiltrated within the premuscle mass (pmm) and the epidermis (e). (G) High power view of c-fms–positive cells from a section of brain at 11.5 dpc shows clear evidence that many labeled cells have engulfed dying cells and contain pyknotic nuclei stained intensely with neutral red. (H) High-power view ofc-fms–positive cell present in the liver shows stellate morphology and intimate association with smaller hematopoietic cells (double arrows).

Localization of cells expressing c-fmsmRNA in the embryo. In all panels, c-fms mRNA was localized by whole mount in situ hybridization as described in Materials and Methods. The blue-purple reaction product indicates sites of expression of the c-fms mRNA. In panels F, G, and H, the stained embryos were embedded, sectioned, and counterstained with neutral red. Bar in panels A through C and E represents 250 μm; in panels D, F through H, 20 μm; heart (*), forelimb (fl), hindlimb (hl), neural tube (nt). (A) Embryo 10.5 dpc. Localization of c-fms mRNA shows a very extensive speckled pattern throughout the embryo. Each spot is a single-labeled cell. Labeled cells are particularly concentrated in the head, around the branchial arches, and along the dorsal midline, although this is not evident in a single focal plane. (B) Embryo 11 dpc. The head of the embryo was removed before staining. At a slightly more advanced stage than (A), the rapid appearance ofc-fms–labeled cells is evident in the liver (l). The fine speckled pattern of labeled cells is more prominent throughout the embryo than in (A), but is less readily demonstrated in a single focal plane. (C) Hindlimb of 12.5 dpc embryo showing accumulation ofc-fms–positive cells at the apical ectodermal ridge (aer) (arrowheads) and infiltration between the digits (arrows). (D) A section through the limb bud of 12.5 dpc embryo showing the positive cells leaving the marginal sinus (ms) and infiltrating the mesenchyme. (E) Whole mount of the head of a hemisected 12.5-dpc embryo. Numerousc-fms–positive cells are lining the ventricular surfaces of the brain, including telencephalon (tel), diencephalon (dic), midbrain (mb), and hindbrain (hb). (F) Section through the body wall of an 11.5-dpc embryo. Numerous c-fms–positive cells accumulate particularly along the dorsal midline (d). In this field, they can be seen to be closely associated with the vascular spaces adjacent to the neural tube, but are also infiltrated within the premuscle mass (pmm) and the epidermis (e). (G) High power view of c-fms–positive cells from a section of brain at 11.5 dpc shows clear evidence that many labeled cells have engulfed dying cells and contain pyknotic nuclei stained intensely with neutral red. (H) High-power view ofc-fms–positive cell present in the liver shows stellate morphology and intimate association with smaller hematopoietic cells (double arrows).

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