Inhibition of biotinylated-LF binding to immobilized CD36 by (A) unlabeled LF or (B) anti-CD36 antibody, 8A6. The CD36-immobilized wells were pretreated with (A) unlabeled LF or 8A6 at the indicated concentration. After removal of pretreatment solution, the microtiter plates were subjected to biotinylated-LF-binding assay. Absorbance at 405 nm (A405) obtained in the absence of FA6 was subtracted from data, and the results are expressed as a percentage of the binding in the absence of inhibitor. Each point represents the mean ± SD of triplicate determinations. The averages of A405 obtained by biotinylated LF binding to CD36 in the absence of unlabeled LF were (A) 0.126 and (B) 0.115, respectively.