Fig. 5.
Fig. 5. Number of week-5 CFC produced in replicate LTC-IC assays initiated at day 10 with cells from individual clones. At day 10, 38 clones grown without MS-5 cells and 65 clones grown with MS-5 were subdivided into 10 replicate wells further kept in standard LTC-IC conditions. CFC were measured in each replicate after 5 weeks. Results for 20 clones with MS-5 cells (right panel, •) and 9 clones without MS-5 (left panel, ○) are shown. Each point represents the number of CFC in one replicate and results for all replicates from one clone are lined on the vertical axis (as indicated by the arrow). Data presented are from one experiment (out of two) initiated with CD34+CD38neg cells.

Number of week-5 CFC produced in replicate LTC-IC assays initiated at day 10 with cells from individual clones. At day 10, 38 clones grown without MS-5 cells and 65 clones grown with MS-5 were subdivided into 10 replicate wells further kept in standard LTC-IC conditions. CFC were measured in each replicate after 5 weeks. Results for 20 clones with MS-5 cells (right panel, •) and 9 clones without MS-5 (left panel, ○) are shown. Each point represents the number of CFC in one replicate and results for all replicates from one clone are lined on the vertical axis (as indicated by the arrow). Data presented are from one experiment (out of two) initiated with CD34+CD38neg cells.

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