The effect of various agents on neutrophil production of ROS. Freshly isolated neutrophils were incubated in the absence (control) or presence of the caspase-8 inhibitor, IETD.fmk (25 μmol/L), the caspase-2 inhibitor, VDVAD.fmk (50 μmol/L), the PKC inhibitor, GF109203X, or an agonistic Fas antibody (500 ng/mL). Relative production of ROS was measured by a flow cytometric assay (DCF-DA) at the indicated time points. Mean ± SE of three separate experiments for VDVAD.fmk and four separate experiments for the rest.